Processo de produção de enxerto ósseo e enxerto ósseo assim obtido
Biotecnologia / Medicina
Producing a null mutant of the URA3 gene of Saccharomyces cerevisiae for metabolizing various sources to produce different industrial metabolites, involves deleting complete copies of the gene using a yeast URA3 integrating cassette
NOVELTY – Producing a null mutant of the URA3 gene of Saccharomyces cerevisiae involves deleting complete copies of the gene using a yeast URA3 integrating cassette. The second copy of the yeast URA3 gene is deleted, and integrated with FGY010 cassette. The fragment homologous repair is used to produce second copy of the URA3 locus FGY020 genome. The Hph cassette containing hphMX4 the marker gene is withdrawn. The yeast genome FGYQ3G is integrated with the core cassette. The genetic markers kanMX4 and KiURA3 are withdrawn by using same fragment of repair. USE – Method for producing null mutant of the URA3 gene of Saccharomyces cerevisiae for metabolizing various sources to produce different industrial metabolites such as alcohol, glycerol, ethylene, propylene and biodiesel hydrocarbons, and for producing alcohol from canade-sugar (all claimed). ADVANTAGE – The method enables to produce a null mutant of the URA3 gene of Saccharomyces cerevisiae with good fermentation performance and excellent adaptation to the industrial conditions.
Main Application Field
D16 (Fermentation industry – including fermentation equipment, brewing, yeast production, production of pharmaceuticals and other chemicals by fermentation, microbiology, production of vaccines and antibodies, cell and tissue culture and genetic engineering.); E17 (Other aliphatics.); H06 (Gaseous and liquid fuels – including pollution control. Chemical aspects of catalytic exhaust systems for cars are included as well as liquid or gaseous fuels of non-petroleum origin e.g. methanol or ethanol-based fuels. Combustion improvement additives for liquid fuels are included (C10L).)
INVENTORS:
DUARTE FABIANA DE MELO
GALZERANI FELIPE
PEREIRA GONÇALO AMARANTE GUIMARÃES
ARGUESO JUAN LUCAS
NETTO OSMAR VAZ DE CARVALHO
MISSAWA SILVIA KAZUE
