NOVELTY – Attenuated Escherichia coli strain is obtained by deleting elpV gene, Hcp gene and IcmF gene, where the strain is deposited under code GSE 21893. The pathogenic stain has nucleobases (SEQ ID NO: 7) and the attenuated stain has nucleobases (SEQ ID NOs: 8, 11, 12). The attenuated stain is amplified by forward and reverse primer (SEQ ID NOs: 9-10). (Sequences not defined here may be found at ftp://ftp.wipo.int/pub/publishedpct sequences/publication). USE – Attenuated Escherichia coli strain for treating septicemia in birds. ADVANTAGE – The attenuated Escherichia coli strain inhibits the mobility of bacteria by inhibiting adenosine triphosphatase that provides energy for the formation of hexameric transmembrane protein. DETAILED DESCRIPTION – INDEPENDENT CLAIMS are included for the following: (1) a vaccine for treating septicemia in birds, which comprises attenuated Escherichia coli strain and pharmaceutically acceptable carrier; and (2) a method for preparing a vaccine for treating septicemia in birds.
B04 (Natural products and polymers. Including testing of body fluids (other than blood typing or cell counting), pharmaceuticals or veterinary compounds of unknown structure, testing of microorganisms for pathogenicity, testing of chemicals for mutagenicity or human toxicity and fermentative production of DNA or RNA. General compositions.); D16 (Fermentation industry – including fermentation equipment, brewing, yeast production, production of pharmaceuticals and other chemicals by fermentation, microbiology, production of vaccines and antibodies, cell and tissue culture and genetic engineering.)