NOVELTY – Bacterin vaccine preparation involves adding catecholamines (adrenaline 10-2000 micromolar) and autoinducers in vaccine culture medium, and then processing the obtained culture medium using quorum sensing bacterial communication system to obtain the desired product, where the catecholamines and autoinducers are added in the vaccine to increase the expression of antigens in enterotoxigenic Escherichia coli. The autoinducer is supernatant of culture medium (5-95%). USE – Method for preparing bacterin vaccine used for controlling colibacillosis in pigs caused by Escherichia coli (all claimed). ADVANTAGE – The method enables to prepare bacterin vaccine that is efficient, and contains catecholamines and autoinducers that ensures improved expression of antigens in enterotoxigenic Escherichia coli.