Process for determining the enzyme glutathione reductase comprises an amperometric sensor for red blood cell hemolysate

Análise de materiais biológicos Backlog Instrumentos

Process for determining the enzyme glutathione reductase comprises an amperometric sensor for red blood cell hemolysate

NOVELTY – A process for determining the activity of the enzyme glutathione reductase in hemolysate comprises depositing oxidized glutathione, NADPH and DTNB (as oxidizing agent of reduced glutathione), all diluted in phosphate/EDTA buffer, on the surface of a lipophilic membrane located on two platinum electrodes, and monitoring the current for 3 minutes after the addition of the sample. USE – In medical equipment. The amperometric sensor may be used to evaluate oxidative stress in medicine and in sports circles. ADVANTAGE – The method gives rapid and reliable results, and may be used outside the laboratory.

Main Application Field

B04 (Natural products and polymers. Including testing of body fluids (other than blood typing or cell counting), pharmaceuticals or veterinary compounds of unknown structure, testing of microorganisms for pathogenicity, testing of chemicals for mutagenicity or human toxicity and fermentative production of DNA or RNA. General compositions.); D16 (Fermentation industry – including fermentation equipment, brewing, yeast production, production of pharmaceuticals and other chemicals by fermentation, microbiology, production of vaccines and antibodies, cell and tissue culture and genetic engineering.); S03 (Scientific Instrumentation); S05 (Electrical Medical Equipment)

INVENTORS:

KUBOTA LAURO TATSUO
ALVES ARMINDO ANTONIO
MACEDO DENISE VAZ DE

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Patent number: BR200202893-A;BR200202893-B1

PATENT STATUS:

GRANTED

FOR ADDITIONAL INFORMATION:

parcerias@inova.unicamp.br

+55 (19) 3521-5207 / 2607

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