NOVELTY – Producing modified Bacillus subtilis LB5a strain, involves constructing a cassette integration and constructing vector integration. The expression vector is integrated with RHFront and TetR, promoter sequences and Pgrac repressor Iacl. RHBack fragment is also integrated with the expression vector in order to transform Bacillus subtilis LB5a strain. USE – Method for producing modified Bacillus subtilis LB5a strain for obtaining surfactin (Claimed). ADVANTAGE – The method enables to produce tetracycline resistant modified Bacillus subtilis LB5a strain, which ensures high growth in the presence of wastes generated during the processing of cassava, and also enables to produce surfactin efficiently. DETAILED DESCRIPTION – An INDEPENDENT CLAIM is also included for a method for producing surfactin, which involves preparation pre-inoculum for fermenting Bacillus subtilis LB5a strain in presence of isopropylthio- beta -galactoside, and then performing centrifugation, precipitation in presence of acid, dissolution, lyophilization and dissolution of the obtained material.
B04 (Natural products and polymers. Including testing of body fluids (other than blood typing or cell counting), pharmaceuticals or veterinary compounds of unknown structure, testing of microorganisms for pathogenicity, testing of chemicals for mutagenicity or human toxicity and fermentative production of DNA or RNA. General compositions.); D16 (Fermentation industry – including fermentation equipment, brewing, yeast production, production of pharmaceuticals and other chemicals by fermentation, microbiology, production of vaccines and antibodies, cell and tissue culture and genetic engineering.)